G5013
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G5013
Sigma
G 418 disulfate salt
potency: ≥450 μg per mg (anhydrous basis)
| Synonym: | Antibiotic G418 |
| CAS Number: | 108321-42-2 |
| Linear Formula: | C20H40N4O10 · 2H2SO4 |
| Molecular Weight: | 692.71 |
| MDL number: | MFCD00058314 |
| PubChem Substance ID: | 24895193 |
Description
| Biochem/physiol Actions | Mode of Action: Blocks polypeptide synthesis by inhibiting protein elongation. For use in the selection and maintenance of eukaryotic cells stably transfected with neomycin resistance genes. |
| Caution | Stable at 37 °C for 8 days. |
| Other Notes | Aminoglycoside antibiotic similar in structure to gentamicin, neomycin and kanamycin. |
| Reconstitution | Aqueous solutions stored frozen at -20 to -70 °C should be stable for approximately six months. |
| Application | Blocks protein synthesis by interfering with ribosomal function. Not normally used as a standard antibiotic, but rather for selection and maintenance of prokaryotic and eukaryotic cells stably transfected with neomycin resistance genes. Resistance is conferred by one of two dominant genes of bacterial origin which can be expressed in eukaryotic cells. The resistance genes are APH (3') II or aminoglycoside phosphotransferase 3'(II) and aminoglycoside phosphotranferase 3' (I) or APH (3') I. The following concentrations of active ingredient may be used as a guide for selection: mammalian cells (400 ug/ml), plants (10 ug/ml), yeasts (500 ug/ml), and dictyostelium (10 ug/ml). Cells have variable resistance and multiplying cells will be affected sooner than those that are not. Cells in log phase may require three to seven days for selection. Varying concentrations should be tested. Additionally, fibroblast contamination in mixed cultures may be suppressed by using 100 ug/ml of the active ingredient for 2 days. |
| Legal Information | Geneticin is a registered trademark of Gibco BRL Life Technologies, Inc. |
Properties
| form | white powder |
| potency | ≥450 μg per mg (anhydrous basis) |
| solubility | H2O: 50 mg/mL |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
References
| reference | Kingston, R, Ausubel, F. et al., Stable transfer of genes into mammalian cells. Short Protocols in Molecular Biology 2nd ed., New York , (1992), 9.14 |
| Canaani, D., and Berg, P., Regulated expression of human interferon β1 gene after transduction in to cultured mouse and rabbit cells. Proc. Natl. Acad. Sci. U. S. A. 79, 5166-5170, (1982) | |
| Southern, P., and Berg, P., Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Biol. 1, 327-341, (1982) | |
| Halaban, R., and Alfano, F., Selective elimination of fibroblasts from cultures of normal human melanocytes. In Vitro Cell. Dev. Biol. 20, 447-450, (1984) | |
| Ursic, D., et al., A new antibiotic with known resistance factors, G418, inhibits plant cells. Biochem. Biophys. Res. Commun. 101, 1031-1037, (1981) | |
| Bar-Nun, S., et al., G-418, an elongation inhibitor of 80 S ribosomes. Biochim. Biophys. Acta 741, 123-127, (1983) |
